2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 2:00 PM - 3:30 PM | UNDERSTANDING THE CONTRIBUTIONS OF MICROBIAL SOURCES TO SURFACE WATER TO INFORM MANAGEMENT OF WATERBORNE PATHOGENS IN WYOMING | Virtual Platform
UNDERSTANDING THE CONTRIBUTIONS OF MICROBIAL SOURCES TO SURFACE WATER TO INFORM MANAGEMENT OF WATERBORNE PATHOGENS IN WYOMING
Pathogen contamination is the leading cause of impairment in American rivers. Most water quality monitoring programs use fecal indicator bacteria (FIB) as a proxy for enteric pathogens, despite uncertainty about their indication of pathogen presence. That uncertainty can be overcome by multi-method microbial monitoring. For example, high-throughput sequencing (HTS, 16S amplicon sequencing) can provide information about fecal sources and microbial communities that standard enumeration methods cannot. We provide critical information about abundance and sources of fecal pollution to water resource managers using culture-based enumeration of Escherichia coli and Enterococcus spp., anti-microbial resistance in FIB isolates, coliphage typing and community-based source tracking via HTS. Antimicrobial resistance against at least one antibiotic was observed in 51% of the 117 E. coli isolates and 16% of the 80 Enterococcus spp. isolates. Resistance to antimicrobials used in animal agriculture such as tetracycline and fluoroquinolones were observed in isolates. Use of molecular methods in conjunction with standard enumeration provides a more complete understanding of microbial communities in lotic environments. These new data will inform resource management plans by providing novel insights on pathogen and bacterial sources for recently listed streams where plans are not established.
- Anthropogenic
- Monitoring
- Stream
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Presenters/Authors
Kelsey Ruehling
(), University of Wyoming, kruehlin@uwyo.edu;
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Harneel Kaur
(), University of Wyoming, hkaur@uwyo.edu;
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Nicolas Blouin
(), University of Wyoming, nblouin@uwyo.edu;
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Bledar Bisha
(), University of Wyoming, Bbisha@uwyo.edu;
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Sarah Collins
(), University of Wyoming, sarah.collins@uwyo.edu;
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