2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/24/2021  |   8:30 AM - 10:30 AM   |  PATTERNS IN MICROBIAL COMMUNITIES IN A LARGE RIVER AND CONNECTED BACKWATER LAKES WITH VARIABLE HYDROLOGY   |  Virtual Platform

PATTERNS IN MICROBIAL COMMUNITIES IN A LARGE RIVER AND CONNECTED BACKWATER LAKES WITH VARIABLE HYDROLOGY

Hydrology strongly influences aquatic ecosystems, with consequences for microbial communities and important processes (e.g., decomposition, phytoplankton productivity). The goal of our study was to examine variation in the microbial community across hydrologically variable systems. Water samples were collected on eight dates in Summer 2018 from four sites in central Illinois, including the Illinois River and three backwater lakes. Water levels at one site (Thompson Lake) were lowered via pumping during the season, allowing comparison of a site with modified hydrology to sites linked more to river hydrology. Water samples were filtered and DNA extracted for 3 size fractions (>20 micron, 3-20 micron, and 0.22-3 micron). Sequencing data targeting different taxa (16S for bacteria, ITS for fungi) were generated using Illumina MiSeq. We found that microbial diversity and composition varied among sites (p < 0.05) and size fractions (p < 0.001), while not over time (p > 0.05). Differences among sites may arise from hydrological and other environmental factors, while differences among fractions suggest communities of particulate-associated microbes differ from free-living. Our findings offer new insights into variation in these microbial communities, with implications for ecosystem functioning and water quality.

  • Biodiversity
  • Ecological dynamics
  • Genomics

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Presenters/Authors

John Marino (), Bradley University, jmarino@fsmail.bradley.edu;


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Alexandra Beanblossom (), Bradley University, abeanblossom@mail.bradley.edu ;


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