2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/20/2019 | 11:00 AM - 11:15 AM | DEVELOPMENT OF QPCR METHODS TO ASSESS THE SPATIAL AND TEMPORAL VARIABILITY OF HARMFUL ALGAL BLOOMS | 254 B
DEVELOPMENT OF QPCR METHODS TO ASSESS THE SPATIAL AND TEMPORAL VARIABILITY OF HARMFUL ALGAL BLOOMS
Cyanobacterial blooms are becoming a greater concern in freshwater systems due to their increasing frequency and potential to produce toxic secondary compounds. Microcystis is a common bloom forming cyanobacteria that is capable of creating harmful algal blooms (HABs) through the production of the hepatotoxin, microcystin. HABs can cause many negative effects to the surrounding environment that include scums on surface waters, hypoxia, or unsafe drinking water. The goal of my project is to develop quantitative polymerase chain reaction (qPCR) methods that allow for early detection and quantification of toxic and non-toxic Microcystis strains to aid in the management of water resources. Water samples were collected from Bear and Muskegon Lakes biweekly between July and September 2017. The samples collected during the summer indicate a much higher proportion of non-toxic to toxic cells in both lakes, which is in accordance with similar findings in the literature. DNA copy numbers of the toxin producing gene were found to be correlated with concentrations of microcystin present in the water column. The development of these qPCR methods will serve as a tool for lake managers to make informed decisions regarding public and environmental health.
- Eutrophication
- Temporal
- Genetics
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Presenters/Authors
Andrew Pyman
(), Grand Valley State University, pymana@mail.gvsu.edu;
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