2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/21/2018 | 2:15 PM - 2:30 PM | BACTERIAL AND FUNGAL SPECIES RESPOND DIFFERENTLY TO LEAF LITTER TYPE: APPLICATIONS OF QUANTITATIVE STABLE ISOTOPE PROBING (QSIP) | 330 A

BACTERIAL AND FUNGAL SPECIES RESPOND DIFFERENTLY TO LEAF LITTER TYPE: APPLICATIONS OF QUANTITATIVE STABLE ISOTOPE PROBING (QSIP)

Quantitative stable isotope probing (qSIP) combined with high throughput sequencing provides a new tool for differentiating active versus inactive microbial taxa throughout leaf litter decomposition. Prior research has demonstrated this technique using 16S rRNA gene sequencing to differentiate active versus inactive bacterial and archeal taxa. Here we demonstrate, for the first time, that qSIP combined with sequencing the internal transcribed spacer region (ITS) of rRNA genes can be used to identify fungal taxa that are actively growing on decomposing leaves. We applied this approach to a litter decomposition experiment in which cottonwood (Populus fremontii and P. angustifolia), ash (Fraxinus velutina ), and oak (Quercus gambelii) leaves were incubated in stream water for up to 24 days. Results show that the majority of fungal taxa found on leaf litter were not active. Analyzing the active taxa revealed significant differences in fungal community composition among leaf types and harvest dates. Additionally we compared bacterial and fungal responses across leaf types to characterize the relative activities of all members of the microbial assemblage during decomposition.

Decomposition
Organic Matter
Isotope

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Presenters/Authors

Michaela Hayer (), Northern Arizona University, michaela.hayer@nau.edu;