2022 Early Hearing Detection & Intervention Virtual Conference

March 13 - 15, 2022

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5/24/2018  |   2:15 PM - 2:30 PM   |  BIOFILM ACCUMULATION MEDIATES THE TRANSPORT OF GENETICALLY-ENGINEERED PROTEIN (CRY1AB) IN EXPERIMENTAL STREAMS   |  330 A

BIOFILM ACCUMULATION MEDIATES THE TRANSPORT OF GENETICALLY-ENGINEERED PROTEIN (CRY1AB) IN EXPERIMENTAL STREAMS

The majority of corn planted in the US is genetically-engineered, expressing insecticidal proteins such as Cry1Ab, in order to prevent crop damage by the European corn borer. As maize detritus from fields enters waterways after harvest, Cry1Ab proteins can also leach into adjacent streams, but the environmental fate of Cry1Ab is not well-studied. To fill this gap, we conducted monthly (June-November) short-term additions of leached Cry1Ab in four 50m experimental streams to estimate Cry1Ab transport and retention. At the onset of our experiments when rocks were bare, we found no evidence of Cry1Ab uptake, but retention steadily increased with benthic biofilm colonization. Overall, Cry1Ab uptake was predicted by epilithon chlorophyll a and filamentous algal cover. Average Cry1Ab removal, expressed as an uptake velocity (vf = 0.059 0.009 mm/s) was comparable to previously reported uptake of labile dissolved organic carbon (mean vf = 0.04 0.008 mm/s). Although Cry1Ab rapidly degrades in stream water, biofilms colonizing inorganic and organic surfaces may influence the distance proteins are transported in lotic systems, making Cry1Ab detection dependent on the ecological context of the stream ecosystems where it is released.

  • Aquatic-terrestrial Linkage
  • Dispersal
  • Hotspot

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Presenters/Authors

Arial Shogren (), Department of Earth and Environmental Sciences, Michigan State University, shogrena@msu.edu;


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Jennifer L. Tank (), University of Notre Dame, tank.1@nd.edu;


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Martha M. Dee (), University of Notre Dame, mdee@nd.edu;


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Shannon Speir (), University of Notre Dame, sspeir@nd.edu;


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Emma Rosi (), Cary Institute of Ecosystem Studies, rosie@caryinstitute.org;


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Scott Egan (), scott.p.egan@rice.edu , Rice University;


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Diogo Bolster (), University of Notre Dame, diogo.bolster.5@nd.edu;


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