2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
5/24/2018 | 2:45 PM - 3:00 PM | GENOMIC AND PHYSIOLOGICAL CHARACTERISTICS OF NOVEL ESCHERICHIA STRAINS ISOLATED FROM FRESH WATER SOURCES | 410 A
GENOMIC AND PHYSIOLOGICAL CHARACTERISTICS OF NOVEL ESCHERICHIA STRAINS ISOLATED FROM FRESH WATER SOURCES
Escherichia coli is commonly viewed as a commensal of the animal gut, but “naturalized” populations of Escherichia found in non-host associated have been observed. Most Escherichia genome sequences come from host-associated isolates, not addressing the potential diversity of strains found in secondary environments, such as freshwater streams and lakes. We produced 104 genome sequences of Escherichia from freshwater sources as part of a longitudinal survey in the Macatawa Watershed, representing a significant increase of genomic information from non-clinical sources. Isolates were compared with available sequenced Escherichia strains using core genome phylogenetic inference. Only 3 strains are related to non-traditional environmental clades, whereas 101 watershed strains are related to traditional E. coli; 46% are members of phylogroup B1. We used in silico methods to identify virulence factors and antibiotic resistance genes. Watershed strains show significantly fewer virulence factors than related clinically derived strains. We determined protein families that distinguish among watershed and reference Escherichia strains within established phylogroups. Physiological experiments testing for metabolic activity of watershed strains revealed activity as low as 2°C. These data expand our knowledge of potentially naturalized E. coli strains found in freshwater environments.
- Microbial
- Bacteria
- Metagenomics
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Presenters/Authors
Amy Olgers
(), Hope College, amy.olgers@hope.edu;
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Jacob Peecher
(), Hope College, jacob.peecher@hope.edu;
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Adam Slater
(), Hope College, adam.slater@hope.edu;
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Shannon Smith
(), Hope College, Shannon.smith@hope.edu;
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Sarah Brokus
(), Hope College, brokus@hope.edu;
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Francesco Moen
(), Hope College, moen@hope.edu;
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Chelsea Payne
(), Hope College, cepayne7@gmail.com;
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Randall Wade
(), Hope College, wade@hope.edu;
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Michael Pikaart
(), Hope College, pikaart@hope.edu;
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Aaron Best
(), Hope College, best@hope.edu;
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