2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
5/21/2018 | 3:00 PM - 3:15 PM | POTENTIAL SYMBIOTIC NITROGEN PROVISIONING BY FRESHWATER INSECT GUT MICROBES THROUGH NITRATE REDUCTION. | 330 A
POTENTIAL SYMBIOTIC NITROGEN PROVISIONING BY FRESHWATER INSECT GUT MICROBES THROUGH NITRATE REDUCTION.
Freshwater insects (FWIs) face comparable nitrogen (N) limitations as their terrestrial equivalents (Elsner et al. 200). However, compared to terrestrial insects, little is known about the role of gut-associated microbiota in alleviating N limitation in FWIs. This is despite the existence of functional feeding group-specific gut microbial assemblages in FWIs (Pechal & Benbow, 2016, Ayayee at al., 2017 in review). Microbial nitrate reduction processes represent possible pathways for symbiotic N provisioning in FWIs. These processes can convert nitrate to nitrous oxide and nitrogen (incomplete denitrification)(Stief et al., 2009) or ammonium (NH4) (assimilatory and dissimilatory nitrate reduction, ANRA and DNRA, respectively). We present herein, the incorporation of 15N into the biomass of FWIs following incubation in filtered stream water or artificial stream water supplemented with labeled potassium nitrate (K15NO3). Uptake is in the form of 15NH4, mediated via the glutamine synthetase (GS)-glutamine:2-oxoglutarate amidotransferase (GOGAT) enzyme complex. Results highlight novel microbial nitrogen provisioning in freshwater insects. Stable isotope and gene expression data from additional studies using only nymphs of the Brown Drake mayfly (Ephemera Simulans), together with inhibitors of both ANRA and DNRA and ammonium assimilation, are pending.
- Bacteria
- Nitrogen
- Invertebrate
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Presenters/Authors
Paul Ayayee
(), University of Wyoming, akwettey@gmail.com;
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Sohini Bhattacharyya
(), Kent State University, sbhatta6@kent.edu;
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Laura Leff
(), Kent State University, lleff@kent.edu;
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