2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
5/22/2018 | 2:30 PM - 2:45 PM | HOW GENE MARKER AND PCR PRIMER CHOICE INFLUENCE DNA METABARCODING OF AQUATIC COMMUNITIES | 420 B
HOW GENE MARKER AND PCR PRIMER CHOICE INFLUENCE DNA METABARCODING OF AQUATIC COMMUNITIES
Molecular genetic tools like DNA barcoding/metabarcoding hold promise for improving our ability to characterize biotic composition, with relevance to detecting invasive species, monitoring species of concern, or describing diversity for bioassessments. To be most effective, these genetic techniques must work across various levels of biodiversity and while DNA barcoding is touted as ‘universally’ applicable, in practice, this is rarely the case. Using data from ballast water, larval fish, lake zooplankton, and stream periphyton communities, we will discuss how decisions about choice of genetic marker and PCR primers influence analyses of aquatic biodiversity, including taxonomic resolution, species composition, and measures of dominance or rarity of component taxa. We will also discuss the challenge of combining data across multiple genetic markers in an effort to provide guidance for future aquatic DNA barcoding research.
- Biodiversity
- Invasive
- Periphyton
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Presenters/Authors
Erik Pilgrim
(), U.S Environmental Protection Agency, pilgrim.erik@epa.gov;
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Aabir Banerji
(), U.S. EPA, banerji.aabir@epa.gov;
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John Darling
(), U.S Environmental Protection Agency, darling.john@epa.gov;
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Chelsea Hatzenbuhler
(), USEPA/NHEERL/MED,ORISE, Duluth, MN, hatzenbuhler.chelsea@epa.gov ;
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Joel Hoffman
(), USEPA/NHEERL/MED, Duluth, MN, hoffman.joel@epa.gov;
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Aaron P. Maloy
(), United States Fish and Wildlife Service, Northeast Fishery Center, aaron_maloy@fws.gov;
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John Martinson
(), U.S Environmental Protection Agency, martinson.john@epa.gov;
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Richard Mitchell
(), U.S. Environmental Protection Agency, mitchell.richard@epa.gov;
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Christy Meredith
(), NRC Fellow, USEPA, MED,Duluth MN, Meredith.Christy@epa.gov;
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Sara Okum
(), USEPA/NERL/SED,ORISE, Cincinnati, OH, okum.sara@epa.gov;
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Anett Trebitz
(), USEPA/NHEERL/MED,Duluth,MN, Trebitz.Anett@epa.gov;
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Lester Yuan
(), U.S. Environmental Protection Agency, yuan.lester@epa.gov;
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