2022 Early Hearing Detection & Intervention Virtual Conference

March 13 - 15, 2022

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5/21/2018 | 10:15 AM - 10:30 AM | HIGH THROUGHPUT SEQUENCING FOR THE STUDY OF MICROBIAL CONTAMINATION OF BEACHES IN THE HURON TO ERIE CORRIDOR: INVESTIGATING DYNAMICS AND POTENTIAL SOURCES | 410 A

HIGH THROUGHPUT SEQUENCING FOR THE STUDY OF MICROBIAL CONTAMINATION OF BEACHES IN THE HURON TO ERIE CORRIDOR: INVESTIGATING DYNAMICS AND POTENTIAL SOURCES

Background: Belle Isle Beach (Detroit) and Sand Point Beach (Windsor) are on either side of the international border, 5 km apart near the Lake St. Clair origin of the Detroit River. Monitoring their microbial levels may be important for managing health risks. Both beaches are affected by Lake St. Clair but differential hydrology and corresponding microbial sources may be different. Methods: We conducted 4-day/week E.coli IDEXX enumeration at both beaches and also filtered water (0.2 um) for Ion Torrent sequencing of ambient bacteria rRNA gene barcodes. Results: While mostly “clean,” three “events” possibly associated with weather yielded high counts simultaneously at both beaches; near-shore counts were higher than waist-deep. Bacterial richness was low in June, becoming more complex with different bacterial communities in July and August than in June. Bacterial communities were distinctly different between the two beaches. Pathogen-related genera were present sporadically, but further analysis is needed to determine if these are pathogenic strains. Conclusion: The beaches are mostly healthy but must be monitored because high-risk events and possible pathogens occur. Further analysis will help define monitoring needs for the future.

Bacteria
Assessment
Bioindicators

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Presenters/Authors

Jeffrey Ram (), Department of Physiology, Wayne State University, jeffram@wayne.edu;