2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
5/20/2019 | 2:30 PM - 2:45 PM | DETECTING WATER QUALITY THRESHOLDS FOR ALGAL BLOOMS USING A SHORT-TERM, MULTI-SPECIES ASSAY | 150 DEF
DETECTING WATER QUALITY THRESHOLDS FOR ALGAL BLOOMS USING A SHORT-TERM, MULTI-SPECIES ASSAY
A strong mechanistic understanding of algal responses to environmental conditions is needed to predict and manage harmful algal blooms (HABs). Typically this understanding is gained with laboratory cultures where growth and biochemical responses can be isolated, or through rigorous field-based correlation studies. Given inherent limitations such as the inability to culture some species, unknown species interactions, and intrinsic variability in field studies, additional approaches are needed to better assess many species. We are developing a Fourier-Transform infrared microspectroscopy based method that can measure biochemical changes in individual algae at the cellular level on a time scale of hours to days. Additionally, target species do not need to be isolated during incubation. We are using the nuisance diatom Didymosphenia geminata and green alga Cladophora, which can both produce abundant biomass that blanket stream bottoms, as model algae. FTIR microspectroscopy is being used to pinpoint water quality thresholds that trigger protein production for cell division, or carbohydrate and lipid production often observed in nutrient-stressed algae. This approach allows a relatively quick assessment of individual species within multi-species incubation, which may provide a more accurate assessment of HAB triggers in streams and lakes.
- Assessment
- Biogeochemistry
- Eutrophication
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Presenters/Authors
Justin Murdock
(), Tennessee Tech University, jnmurdock@tntech.edu;
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Samuel Day
(), Tennessee Tech University, sjday42@students.tntech.edu;
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