2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
6/08/2017 | 12:00 PM - 12:15 PM | PATHOGENIC BACTERIA AND ANTIBIOTIC RESISTANCE IN SOUTH DAKOTA SURFACE WATERS | 305B
PATHOGENIC BACTERIA AND ANTIBIOTIC RESISTANCE IN SOUTH DAKOTA SURFACE WATERS
Between 2003 and 2012, there were 390 E. coli O157 outbreaks in the US, including 15 waterborne outbreaks that sickened 154 people, including one fatality. In 2012, South Dakota ranked third for STEC infection and first for E. coli O157 infection. Additionally, South Dakota experienced the highest number of recorded STEC infections in history in 2016 with an 86% increase over the five-year average. Routine fecal contamination testing does not take into account the bacterial pathogenicity. Rapid Creek, Skunk Creek, and the Big Sioux River have exceeded standards for fecal coliforms and/or E. coli. We have developed and applied a pathogenicity metric to assay the disease-causing potential of organisms in impaired surface water. From total DNA isolated from water samples, we detected genes encoding two Shiga toxins (stx1/VT1; stx2/VT2), an intimin (eaeA), an enterohemolysin (ehxA), and an enteroinvasin (einV). We screened a subset of our samples for antibiotic resistance genes finding Amp-C, Tet-C , Str-A , and a Van-A gene as well as multiply resistant organisms. These results indicate a higher potential health risk than does measuring E. coli alone.
- C30 Molecular Ecology
- C36 Water Resource Management
- C04 Microbial Ecology
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Presenters/Authors
Lisa Kunza
(), South Dakota School of Mines and Technology, lisa.kunza@sdsmt.edu;
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Kelsey Murray
(), South Dakota School of Mines and Technology, kelsey.murray@mines.sdsmt.edu;
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Ashley Preston
(), South Dakota School of Mines and Technology, ashley.ditlev@mines.sdsmt.edu;
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Linda DeVeaux
(), South Dakota School of Mines and Technology, linda.deveaux@sdsmt.edu;
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