2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
5/24/2021 | 2:00 PM - 3:30 PM | DEGRADATION OF ENVIRONMENTAL DNA WITH MULTIPLE EDNA SOURCES IN FRESHWATER AND SEAWATER | Virtual Platform
DEGRADATION OF ENVIRONMENTAL DNA WITH MULTIPLE EDNA SOURCES IN FRESHWATER AND SEAWATER
Environmental DNA (eDNA) methods have been developed to detect organisms’ distributions abundance/biomass in various environments. eDNA degradation is critical for eDNA evaluation, while, the dynamics and mechanisms of eDNA degradation are largely unknown. We conducted the degradation experiments using freshwater and sea water and a meta-analysis. Firstly, we experimentally evaluated the degradation rates of eDNA derived from multiple sources, including fragmental DNA (IPC), free cells from Oncorhynchus kisutch, and the resident fish species of pond and sea. Our results showed that eDNA derived from both cells and IPC decreased exponentially in the both pond and sea water samples. The degradation of eDNA from the resident fish species showed similar behavior to the cell-derived eDNA.
As a meta-analysis, we complied the degradation rates of eDNA from 28 studies. We also collected the related factors. Our results suggested that water temperature and PCR amplicon lengths of the measured DNA. were significantly related to the degradation rate of eDNA using 95% quantile models. From the simulation based on the 95% quantile model, we predicted the maximum degradation rate of eDNA in various combinations of water temperature and PCR amplicon length.
- Conservation
- Decomposition
- Genomics
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Presenters/Authors
Tatsuya Saito
(), University of Hyogo, tatwoeight630@icloud.com;
ASHA DISCLOSURE:
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Nonfinancial -
Hideyuki Doi
(), University of Hyogo, hideyuki.doi@icloud.com;
ASHA DISCLOSURE:
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Nonfinancial -