2022 Early Hearing Detection & Intervention Virtual Conference

March 13 - 15, 2022

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6/06/2017  |   2:15 PM - 2:30 PM   |  META-ANALYSIS OF NUTRIENT DIFFUSING SUBSTRATE EXPERIMENTS IN RUNNING WATERS: INFLUENCE OF EXPERIMENTAL DESIGN, ENVIRONMENTAL FACTORS, AND GEOGRAPHY   |  306A

META-ANALYSIS OF NUTRIENT DIFFUSING SUBSTRATE EXPERIMENTS IN RUNNING WATERS: INFLUENCE OF EXPERIMENTAL DESIGN, ENVIRONMENTAL FACTORS, AND GEOGRAPHY

Algal growth is often limited by nitrogen and phosphorus availability. For over 30 years, investigators have conducted nutrient diffusing substrata (NDS) experiments to quantify algal nutrient limitation in rivers and streams. Previous meta-analyses of NDS have shown that algae are often co-limited by nitrogen and phosphorus, and that in-stream nutrients are weakly predictive of limitation. However, no meta-analysis has comprehensively addressed the experimental, environmental, and geographic covariates affecting nutrient limitation results. We reviewed the literature and extracted data for algal biomass effect sizes (n = 649 experiments). We built meta-regression models to identify important controls on NDS results and gain insights about algal nutrient limitation patterns over space and time. Experimental factors such as NDS chemical compound, chemical concentration, and experimental length significantly affected phosphorus limitation and provided insight into potential mechanisms for phosphorus inhibition. Environmental variables affecting NDS results included in-stream nutrients, light, temperature, discharge, and stream velocity. Land use, ecoregion, and season also explained trends in nutrient limitation. Based on our findings, we provide recommendations for improving experimental design and pursuing fruitful research using NDS.

  • C01 Algae
  • C32 Primary and Secondary Production
  • C10 Biogeochemistry

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Presenters/Authors

Whitney Beck (), Environmental Protection Agency, Beck.Whitney@epa.gov;


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Amanda Rugenski (), University of Georgia, atrugenski@gmail.com;


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LeRoy Poff (), Colorado State University, n.poff@rams.colostate.edu;


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