2022 Early Hearing Detection & Intervention Virtual Conference
March 13 - 15, 2022
6/08/2017 | 9:00 AM - 9:15 AM | IMPACTS OF ENVIRONMENTAL CONCENTRATIONS OF TRICLOSAN ON COMPOSITION, FUNCTION, AND ANTIMICROBIAL RESISTANCE IN EPILITHIC BIOFILMS | 305B
IMPACTS OF ENVIRONMENTAL CONCENTRATIONS OF TRICLOSAN ON COMPOSITION, FUNCTION, AND ANTIMICROBIAL RESISTANCE IN EPILITHIC BIOFILMS
Triclosan is the active ingredient in antibacterial soaps that can reach detectable levels in waterways and is expected to have an impact on microbial-mediated ecosystem processes. We assessed the effect of triclosan on epilithic biofilms collected from Cattaraugus Creek, western New York. Biofilms were incubated for 3 weeks at four triclosan concentrations (0.0, 0.1, 1.0 and 10 ppb), representing the full range of concentrations observed in rivers and streams. Biofilm condition (chlorophyll a and AFDM) and function (photosynthesis, respiration, and extracellular enzyme activities) were measured post incubation. Triclosan significantly reduced biofilm chlorophyll content and autotrophic index relative to control, but did not appear to affect measured biofilm function. Interestingly, bacteria from the biofilms did not show triclosan resistance regardless of the treatment level. Subsequent assays indicate that culturable bacteria from the biofilms did not show reduced growth until triclosan concentrations reached 100 ppb. Our data suggests that field concentrations of triclosan may not be sufficient to reduce biofilm growth and function in rivers and streams.
- C10 Biogeochemistry
- C01 Algae
- C04 Microbial Ecology
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Presenters/Authors
Jonathan O'Brien
(), Canisius College, obrien46@canisius.edu;
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David Kerling
(), Canisius College, kerling1@canisius.edu;
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