2022 Early Hearing Detection & Intervention Virtual Conference

March 13 - 15, 2022

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6/07/2017 | 9:00 AM - 9:15 AM | USE OF ENVIRONMENTAL DNA (eDNA) AND THE ELECTRON TRANSPORT SYSTEM (ETS) ASSAY TO ASSESS POPULATIONS OF INVASIVE Orconectes virilis IN THE SOUTHEASTERN UNITED STATES | 305A

USE OF ENVIRONMENTAL DNA (eDNA) AND THE ELECTRON TRANSPORT SYSTEM (ETS) ASSAY TO ASSESS POPULATIONS OF INVASIVE Orconectes virilis IN THE SOUTHEASTERN UNITED STATES

The virile crayfish (Orconectes virilis), is an upper mid-west North American species that has been broadly introduced, with southernmost introductions in the Cahaba River of Alabama. To better understand its current extent and predict future expansion, we are taking a two-pronged approach using eDNA techniques to assess presence/absence in streams, and ETS assays to assess thermal tolerance of the invader relative to congeneric natives. To date, we have developed and optimized general quantitative PCR (qPCR) markers to detect the presence of Orconectes spp. and specific qPCR markers to detect O. virilis and native O. erichsonianus at the species level. We have also developed and refined protocols using the ETS assay to assess differences in thermal tolerance between aquatic species. We are currently surveying Cahaba drainage streams for presence/absence of invasive and native crayfish and comparing thermal tolerances among O. virilis and native Orconectes spp. Results will be of great use in identifying streams at high-risk for future invasions, and native species likely to be impacted by the invader.

C26 Invasive Species
C03 Invertebrates
S12 Challenges, insights, and opportunities in crayfish conservation and ecology

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Presenters/Authors

Hisham Abdelrahman (), School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, hisham@auburn.edu;