EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/24/2018 | 2:45 PM - 3:00 PM | GENOMIC AND PHYSIOLOGICAL CHARACTERISTICS OF NOVEL ESCHERICHIA STRAINS ISOLATED FROM FRESH WATER SOURCES | 410 A
GENOMIC AND PHYSIOLOGICAL CHARACTERISTICS OF NOVEL ESCHERICHIA STRAINS ISOLATED FROM FRESH WATER SOURCES
Escherichia coli is commonly viewed as a commensal of the animal gut, but “naturalized” populations of Escherichia found in non-host associated have been observed. Most Escherichia genome sequences come from host-associated isolates, not addressing the potential diversity of strains found in secondary environments, such as freshwater streams and lakes. We produced 104 genome sequences of Escherichia from freshwater sources as part of a longitudinal survey in the Macatawa Watershed, representing a significant increase of genomic information from non-clinical sources. Isolates were compared with available sequenced Escherichia strains using core genome phylogenetic inference. Only 3 strains are related to non-traditional environmental clades, whereas 101 watershed strains are related to traditional E. coli; 46% are members of phylogroup B1. We used in silico methods to identify virulence factors and antibiotic resistance genes. Watershed strains show significantly fewer virulence factors than related clinically derived strains. We determined protein families that distinguish among watershed and reference Escherichia strains within established phylogroups. Physiological experiments testing for metabolic activity of watershed strains revealed activity as low as 2°C. These data expand our knowledge of potentially naturalized E. coli strains found in freshwater environments.
- Microbial
- Bacteria
- Metagenomics
Presentation:
This presentation has not yet been uploaded.
Handouts:
Handout is not Available
Transcripts:
CART transcripts are NOT YET available, but will be posted shortly after the conference
Presenters/Authors
Amy Olgers
(), Hope College, amy.olgers@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Jacob Peecher
(), Hope College, jacob.peecher@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Adam Slater
(), Hope College, adam.slater@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Shannon Smith
(), Hope College, Shannon.smith@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Sarah Brokus
(), Hope College, brokus@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Francesco Moen
(), Hope College, moen@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Chelsea Payne
(), Hope College, cepayne7@gmail.com;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Randall Wade
(), Hope College, wade@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Michael Pikaart
(), Hope College, pikaart@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Aaron Best
(), Hope College, best@hope.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -