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5/22/2018 | 9:45 AM - 10:00 AM | EFFECTS OF FIELD SAMPLING DESIGN ON ENVIRONMENTAL DNA PERFORMANCE FOR FISH, ZOOPLANKTON, AND ZOOBENTHOS IN TEMPERATE LAKES | 420 B

EFFECTS OF FIELD SAMPLING DESIGN ON ENVIRONMENTAL DNA PERFORMANCE FOR FISH, ZOOPLANKTON, AND ZOOBENTHOS IN TEMPERATE LAKES

We evaluate how depth and location of environmental DNA (eDNA) water samples differentially affects performance of meta-barcoding for diverse freshwater taxa including fish, zooplankton, and zoobenthos in temperate lakes. We took eDNA water samples in 12 Michigan lakes, stratified as three nearshore and three offshore random locations. At each of these six locations per lake, we took a 250 ml surface water sample, and a 250 ml sample from just above the lake bed using a Van Dorn sampler. We filtered eDNA from water samples and Illumina-sequenced gene fragments to characterize fish, zooplankton, and zoobenthos communities. eDNA-estimated species richness was greater for zooplankton than zoobenthos regardless of sample location or depth across lakes. For fish, eDNA results differed considerably between lakes, but location and depth of water samples within lakes had little effect on characterizing communities. Alternatively, for zooplankton, eDNA results were relatively similar between our lakes, but deep water and offshore samples were consistently different from nearshore and shallow eDNA samples within lakes. We demonstrate that sampling design for eDNA meta-barcoding should account for the target taxonomic group in temperate lakes.

Invertebrate
Vertebrate
Community

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Presenters/Authors

Eric Larson (), University of Illinois, erlarson@illinois.edu;