EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/21/2019 | 2:00 PM - 2:15 PM | SPATIOTEMPORAL MICROBIOME ASSEMBLY AND METABOLISM IN HYDROLOGICALLY-CONNECTED MICROBIOMES | 150 G
SPATIOTEMPORAL MICROBIOME ASSEMBLY AND METABOLISM IN HYDROLOGICALLY-CONNECTED MICROBIOMES
Subsurface groundwater-surface water mixing zones (hyporheic zones) have enhanced microbial metabolism, but assembly processes governing hyporheic microbiome composition remain poorly understood. Here, we investigated (a) biogeographical patterns in attached and waterborne microbiomes in three hydrologically-connected areas; (b) assembly processes that generated these patterns; and (c) spatiotemporal relationships between assembly processes and microbial metabolism. All microbiomes remained dissimilar through time, and we demonstrated a pronounced impact of deterministic assembly in all microbiomes. Additionally, we show seasonal shifts from heterotrophy to autotrophy associated with increases in groundwater discharge. The abundance of one cluster of organisms increased with active biomass and respiration, revealing organisms that may strongly influence hyporheic biogeochemistry. Finally, we demonstrate that multiple selective pressures—imposed by sediment and porewater physicochemistry—integrate to generate changes in microbiome composition at distinct timescales among habitat types. Based on our results, we present a conceptual model in which metabolism increases when oscillating (hydrologic) selective pressures oppose stable (sediment) selection; and in which organic carbon concentrations during surface water intrusion supports heterotrophic metabolism. Our research provides new opportunities to enhance microbially-explicit ecosystem models describing hyporheic zone biogeochemistry and its influence over riverine ecosystem function.
- Microbial
- Metabolism
- Hyporheic
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Presenters/Authors
Emily Graham
(), Pacific Northwest National Laboratory, emily.graham@pnnl.gov;
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Alex Crump
(), University of Idaho, acrump@uidaho.edu;
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C. Tom Resch
(), Pacific Northwest National Laboratory, tom.resch@pnnl.gov;
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Sarah Fansler
(), Pacific Northwest National Laboratory, sarah.fansler@pnnl.gov;
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Evan Arntzen
(), Pacific Northwest National Laboratory, evan.arntzen@pnnl.gov;
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James Stegen
(), Pacific Northwest National Laboratory, james.stegen@pnnl.gov;
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