EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/21/2019 | 9:00 AM - 9:15 AM | ESTABLISHING DIDYMOSPHENIA GEMINATA CELLULAR RESPONSE THRESHOLDS TO WATER QUALITY CHANGES USING FOURIER-TRANSFORM INFRARED (FTIR) MICROSPECTROSCOPY | 150 DEF
ESTABLISHING DIDYMOSPHENIA GEMINATA CELLULAR RESPONSE THRESHOLDS TO WATER QUALITY CHANGES USING FOURIER-TRANSFORM INFRARED (FTIR) MICROSPECTROSCOPY
The diatom, Didymosphenia geminata, or Didymo, is considered a nuisance species in coldwater streams across the world, forming thick mats that can blanket stream substrate and homogenize benthic habitat. Low phosphorous conditions (<2 µg/L) have been linked to stalk production; however, new research suggests that other water quality parameters can be important in mat formation in some regions. Standard laboratory bioassays for determining D. geminata cell response thresholds to water quality is difficult because it does not survive long in laboratory conditions. We are developing a method that requires short-term incubations (hours to days) and determines cellular level responses to changing water conditions. Fourier-transform infrared (FTIR) microspectroscopy is used to determine the changes in relative protein, lipid, and carbohydrate concentrations that proceed both cell division and stalk production. Specific biomarkers of interest include carbohydrate increases before stalk production and protein increases before cell division. This new cellular level approach may be an important step towards understanding the mechanisms of D. geminata mat formation. Additionally, this FTIR protocol can be broadly applicable to detecting important triggers of growth and/or toxin production in other harmful and nuisance algae species.
- Assessment
- Water Quality
- Nutrients
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Presenters/Authors
Samuel Day
(), Tennessee Tech University, sjday42@students.tntech.edu;
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Justin Murdock
(), Tennessee Tech University, jnmurdock@tntech.edu;
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