EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021

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6/06/2017 | 2:45 PM - 3:00 PM | GENE EXPRESSION AND METABOLOMICS REVEAL PHYSIOLOGICAL MECHANISMS UNDERLYING THERMAL EFFECTS IN MAYFLY NEOCLOEN TRIANGULIFER | 306C

GENE EXPRESSION AND METABOLOMICS REVEAL PHYSIOLOGICAL MECHANISMS UNDERLYING THERMAL EFFECTS IN MAYFLY NEOCLOEN TRIANGULIFER

Temperature dictates the performance and distributions of aquatic insects. Life history data show that the mayfly Neocloen triangulifer generally follows the temperature size rule, with faster growth rate, smaller body size and less fecundity at warmer temperatures. However, the physiological mechanisms underlying thermal effects remains unclear. Here we attempted to link physiological processes to life history outcomes in N. triangulifer by studying gene expression and metabolomics. We reared N. triangulifer at different static temperatures (22-30°C, 2°C interval) from hatching eggs to mature larvae. Larvae fail to reach adulthood when reared at 30°C.We found no change in hypoxia responsive genes in larvae exposed to thermal stress. Metabolomics data showed decrease of several lipids and acylcarnitines in larvae, suggesting thermal stress largely acts upon bioenergetic pathways. We also observed an increase in tissue histamines, which may indicate larvae likely reduce food intake at upper thermal limits. Collectively, these data suggest that rather than oxygen limitation, energetic challenges and metabolic maintenance costs more likely underlie the observed response of life history to temperature.

C29 Life Histories
C15 Population Ecology
C20 Climate Change

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Presenters/Authors

Hsuan Chou (), North Carolina State University, hchou2@ncsu.edu;