EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/25/2021 | 8:30 AM - 10:30 AM | DETECTION OF PER- AND POLYFLUOROALKYL SUBSTANCES (PFAS) IN GREAT LAKES FISH USING A NOVEL TECHNOLOGY | Virtual Platform
DETECTION OF PER- AND POLYFLUOROALKYL SUBSTANCES (PFAS) IN GREAT LAKES FISH USING A NOVEL TECHNOLOGY
Per- and polyfluoroalkyl substances (PFAS) are man-made fluorinated organic chemical compounds resistant to degradation, making them extremely persistent in the environment and able to bioaccumulate in wildlife and humans. Traditional methods of PFAS analysis (LC-MS/MS) are time-consuming and expensive, but we applied a novel low-cost alternative in particle-induced gamma-ray emission spectroscopy (PIGE) to measure fluorine in fish tissue. PIGE is a rapid screening method that measures total organic fluorine in a sample as a surrogate for the 1000s of possible PFAS compounds in the environment. Our objectives for this study were: (1) determine the relationship between PIGE and traditional LC-MS/MS for fish tissues and (2) determine whether total fluorine in Lake Michigan sportfish differs across species, gender, and size (total length). Results show that Lake Michigan fish have elevated total organic fluorine concentrations compared to fish from Alaska with lower environmental fluorine exposure. Overall, our method development allows for rapid estimation of PFAS in fish as a screening tool from which selected samples can undergo analysis for specific compounds with LC-MS/MS. Our results contribute to a better understanding of the extent of PFAS contamination in aquatic food webs.
- Food webs
- Interdisciplinary
- Anthropogenic
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Presenters/Authors
Whitney Conard
(), Department of Biological Sciences, University of Notre Dame, wconard@nd.edu;
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Sarah Klepinger
(), University of Notre Dame, Biological Sciences, skleping@nd.edu;
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Graham Peaslee
(), University of Notre Dame, gpeaslee@nd.edu;
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Meghanne Tighe
(), University of Notre Dame, Department of Chemistry and Biochemistry, mtighe@nd.edu;
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Heather Whitehead
(), University of Notre Dame, Department of Chemistry and Biochemistry, hwhitehe@nd.edu;
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Alison Zachritz
(), University of Notre Dame, Department of Biological Sciences, azachrit@nd.edu;
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Gary Lamberti
(), University of Notre Dame, glambert@nd.edu;
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