EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/22/2018 | 2:45 PM - 3:00 PM | COMPARISON OF ELECTROFISHING AND ENVIRONMENTAL DNA DETECTION METHODOLOGIES IN SMALL COLD WATER STREAMS | 420 B
COMPARISON OF ELECTROFISHING AND ENVIRONMENTAL DNA DETECTION METHODOLOGIES IN SMALL COLD WATER STREAMS
Use of eDNA can provide insight into fish biodiversity surveys when electrofishing may not be feasible. However, studies that directly compare the data obtained from traditional techniques to those using eDNA are needed. The two primary eDNA detection techniques are quantitative PCR (qPCR) and metabarcoding. qPCR is used for the targeted detection of a specific species, while metabarcoding is used for assessment of the broader species community. Here we evaluated the effectiveness of qPCR and metabarcoding for species detection relative to electrofishing. For five small (<25 cfs) coldwater streams, no differences were found in the relative abundance measures obtained using qPCR, metabarcoding, and electrofishing. In all cases, species data recovered using the various techniques were highly correlated (Pearson r > 0.88). These data suggest that similar measures of presence/absence and relative abundance can be obtained using eDNA detection techniques in small, relatively non-complex stream habitats as compared to that of electrofishing. Additional studies are necessary to evaluate the repeatability of this work and whether similar results can be obtained from a wider diversity of habitat types with increasing species complexity.
- Great Lakes
- Fish
- Metagenomics
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Presenters/Authors
Aaron P. Maloy
(), United States Fish and Wildlife Service, Northeast Fishery Center, aaron_maloy@fws.gov;
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Christopher B. Rees
(), United States Fish and Wildlife Service, Northeast Fishery Center, Christopher_Rees@fws.gov;
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Henry R. Quinlan
(), United States Fish and Wildlife Service, Ashland Fish and Wildlife Conservation Office, Henry_Quinlan@fws.gov;
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Mark J. Brouder
(), United States Fish and Wildlife Service, Ashland Fish and Wildlife Conservation Office, mark_brouder@fws.gov;
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Meredith L. Bartron
(), United States Fish and Wildlife Service, Northeast Fishery Center, meredith_bartron@fws.gov;
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