EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/24/2021 | 8:30 AM - 10:30 AM | PHYLOGENETIC ANALYSIS OF PHAGE COMMUNITY DIVERSITY USIGN PHOH AND THYMIDYLATE SYNTHASE AS SIGNATURE GENES | Virtual Platform
PHYLOGENETIC ANALYSIS OF PHAGE COMMUNITY DIVERSITY USIGN PHOH AND THYMIDYLATE SYNTHASE AS SIGNATURE GENES
Bacteriophages are key regulators of microbial communities. Because Caulobacter crescentus is a common freshwater bacterium, we compared genomic sequences of novel caulophage from New Jersey lakes to each other and to previously described caulophages. We determined that all of our New Jersey phages are Phicbkviruses, a previously described group. Caulophage community diversity was characterized by analyzing phoH and thymidylate synthase (TSase) as signature genes. For both genes, the predicted phage amino acid sequences were distinct from the host sequences. In the TSase phylogenetic tree, the phages associated into six distinct clusters. Some New Jersey phages fell into a known Phicbkvirus clade containing phage isolated from Texas and South Carolina, but the others distributed into two novel clusters distinct from known Phicbkviruses. The phoH tree was similar to that of TSase. However, in phage BL10 the gene is more similar to phoH from C. crescentus than to any of the phage sequences. The most recent common ancestor of all six Phicbkvirus clades likely carried phoH and TSase genes originating from an organism other than Caulobacter, and presumably BL10 replaced its phoH gene via a horizontal transfer event involving the Caulobacter host.
- Genomics
- Microbial ecology
- Biological interactions
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Presenters/Authors
Ana Cruz
(), Rowan University, cruzan26@students.rowan.edu;
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Alisha Vincent
(), Rowan University, vincen86@students.rowan.edu;
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