EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021
(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)
5/22/2018 | 2:15 PM - 2:30 PM | MONITORING AQUATIC INVASIVE SPECIES THROUGH MULTI-MARKER METABARCODING: HOW DOES ENVIRONMENTAL DNA COMPARE TO ICHTHYOPLANKTON TOW SAMPLING? | 420 B
MONITORING AQUATIC INVASIVE SPECIES THROUGH MULTI-MARKER METABARCODING: HOW DOES ENVIRONMENTAL DNA COMPARE TO ICHTHYOPLANKTON TOW SAMPLING?
Ichthyoplankton tows provide one of the earliest possible detections of aquatic invasive species by collecting larval fish and eggs. However, ichthyoplankton tows can be costly, time consuming, and taxonomic identification of the larvae and eggs is challenging. Individual genetic identification of a larvae or egg can confirm visual identification when there is uncertainty, but the process is slow and tissue destructive. We developed a multi-marker sequencing (metabarcoding) method that can identify species of a fish community without destructive sampling or visually identifying each larvae or egg. By using an eDNA approach, non-destructive genetic identification of the entire tow is possible, allowing for specimen recovery if an invasive species is positively identified in a tow. We compared how accurately species identification can be determined by collecting DNA from ethanol used to preserve tow samples and using multiple genetic markers to identify the species composition of the tow. We also determined if environmental DNA in water collected simultaneously with the ichthyoplankton tow provides similar fish community data as the tow itself.
- Invasive
- Great Lakes
- Genetics
Presentation:
This presentation has not yet been uploaded.
Handouts:
Handout is not Available
Transcripts:
CART transcripts are NOT YET available, but will be posted shortly after the conference
Presenters/Authors
Katie Bockrath
(), USFWS, katherine_bockrath@fws.gov;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Maren Tuttle-Lau
(), USFWS, maren_tuttle-lau@fws.gov;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
S.Grace McCalla
(), USGS, smccalla@usgs.gov;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Yer Lor
(), USGS, ylor@usgs.gov;
ASHA DISCLOSURE:
Financial -
Nonfinancial -