EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021

(Virtually the same conference, without elevators, airplane tickets, or hotel room keys)

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5/22/2019  |   10:00 AM - 10:15 AM   |  ONE FISH, TWO FISH: eDNA AS A QUANTITATIVE TOOL   |  254 B

ONE FISH, TWO FISH: eDNA AS A QUANTITATIVE TOOL

Environmental DNA (eDNA) is an emerging tool for the detection of rare and elusive aquatic species with accelerating application in invasive species management and conservation of endangered species. The next step for this emerging tool is quantitative: relating copy numbers of DNA recovered from the environment to abundance or biomass of a target species. Here, we test the quantitative utility of a single-species eDNA assay for an inland fishery in northern Wisconsin (USA). Real-Time quantitative PCR (qPCR) results reveal significant positive relationships between traditional estimates of walleye (Sander vitreus) density and eDNA copy number across 25 lakes. This study supports eDNA’s potential as a quantitative tool, demonstrates the molecular method’s ecological relevance beyond questions of presence/absence, and could inform broader active management of this important walleye fishery.

  • Fish
  • Management
  • Monitoring

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Presenters/Authors

Michael Spear (), University of Wisconsin Madison, Center for Limnology, mspear2@wisc.edu;


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Holly Embke (), University of Wisconsin Madison, Center for Limnology, hembke@wisc.edu;


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Patrick Krysan (), University of Wisconsin Madison, Genome Center, pjkrysan@wisc.edu;


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Jake M. Vander Zanden (), University of Wisconsin Madison, Center for Limnology, mjvanderzand@wisc.edu;


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