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5/24/2018  |   2:30 PM - 2:45 PM   |  TEMPORAL AND SPATIAL VARIATION IN PATTERNS OF BACTERIAL COMMUNITY COMPOSITION AND RELATED METABOLIC FUNCTIONS IN SPRING WATER   |  410 A

TEMPORAL AND SPATIAL VARIATION IN PATTERNS OF BACTERIAL COMMUNITY COMPOSITION AND RELATED METABOLIC FUNCTIONS IN SPRING WATER

Groundwater, which comprises 94 % of all usable freshwater, is one of the world's most extracted renewable resources for agricultural, industrial and domestic purposes. Springs are defined as the ecotone linking surface with groundwater, and have been used as drinking water resources since ancient times. Because of their small size, springs are thought to be sensitive to disturbances such as water abstraction and nutrient inputs. Therefore the aim of our study was to investigate the influence of abiotic variables on the composition and diversity of bacterial communities in springs, as well as to test whether communities differ regarding metabolic functions. We isolated total DNA from water samples collected at five sampling sites at four time points, sequenced the bacterial 16S rRNA gene using high-throughput sequencing, and predicted the metabolic functions of the recovered operational taxonomic units. Of the fifteen abiotic variables that we measured, electric conductivity best explained variation in both microbial community patterns and metabolic functions. Furthermore, metabolic functions and microbial community composition differed according to temporal rather than spatial sampling, indicating a fast community turnover and supporting the idea that spring communities are potentially vulnerable to disturbance.

  • Function
  • Groundwater
  • Disturbance

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Presenters/Authors

Karsten Karczewski (), University of Muenster, k.karczewski@gmx.de;


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Elisabeth I. Meyer (), University of Münster, Institute for Evolution and Biodiversity, Department of Limnology, Hüfferstr. 1, D-48149 Münster, meyere@uni-muenster.de;


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