EARLY HEARING DETECTION AND INTERVENTION VIRTUAL CONFERENCE
MARCH 2-5, 2021

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5/22/2018  |   11:30 AM - 11:45 AM   |  USE OF METABARCODING OF ENVIRONMENTAL DNA SAMPLES TO ASSESS FRESHWATER MUSSEL POPULATION RESTORATION EFFORTS IN THE CLINCH RIVER   |  420 B

USE OF METABARCODING OF ENVIRONMENTAL DNA SAMPLES TO ASSESS FRESHWATER MUSSEL POPULATION RESTORATION EFFORTS IN THE CLINCH RIVER

The Clinch River (Virginia and Tennessee) is one of the most biologically diverse freshwater systems in the country. Known for its high species richness of freshwater mussels, the system contains 46 extant species, including 20 federally listed species. Declines in these diverse mussel communities are linked to anthropogenic alteration of habitat and water quality. In 1998, a large chemical spill killed more than 7,000 mussels, necessitating habitat restoration as well as captive breeding and release of mussels in the early 2000’s. Monitoring of this system is ongoing to better understand effects of these disturbances and subsequent restoration efforts. Non-invasive environmental DNA (eDNA) sampling may become a useful tool for such monitoring efforts. To investigate the utility of eDNA metabarcoding to detect and describe local mussel communities, we collected water samples near well characterized freshwater mussel beds in the Clinch River. We present results from our 2017 water samples and relate community structure obtained from eDNA sampling to known species composition at each site. Finally, we also address ways to improve the use of eDNA metabarcoding for monitoring of freshwater taxa.

  • Metagenomics
  • Monitoring
  • Conservation

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Presenters/Authors

Katy Klymus (), U.S.Geological Survey, kklymus@usgs.gov;


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Catherine Richter (), Columbia Environmental Research Center, USGS, CRichter@usgs.gov;


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Nathan Thompson (), U.S. Geological Survey, nthompson@usgs.gov;


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Jess Jones (), U.S. Fish and Wildlife Service, Jess_Jones@fws.gov;


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