2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/20/2019 | 12:00 PM - 12:15 PM | AN INVESTIGATION OF LINKED ELEMENTAL CYCLES USING DUAL ISOTOPE EXPERIMENTS AND CONSTRAINT-BASED OPTIMIZATION MODELING | 250 AB
AN INVESTIGATION OF LINKED ELEMENTAL CYCLES USING DUAL ISOTOPE EXPERIMENTS AND CONSTRAINT-BASED OPTIMIZATION MODELING
Conceptualizing and modeling multiple interactive elemental cycles remains a challenge in the field of biogeochemistry, even at the scale of experimental mesocosms. Considering stoichiometric links among multiple elemental cycles is critical to predicting the outcomes from a broad spectrum of potentially relevant biogeochemical processes. Measuring and modeling multiple elemental cycles requires consideration of associated environmental signals, such as temporal variation in metabolite abundances and isotopic signatures. Here, we describe a combined experimental and modeling approach that leverages system constraints imposed by stoichiometry and thermodynamics in addition to time series datasets of both metabolite concentrations and stable isotope ratios. We present results from a set of mesocosm experiments in which carboys filled with stream water and gravel were amended with two stable isotope tracers (either 13C-enriched CH3COONa and 15N-enriched NaNO3 or 13C-enriched NaHCO3 and 15N-enriched NH4Cl). The carboy mesocosms were incubated in the dark with no headspace and sampled periodically for 7 days. We use a constraint-based optimization model generated by the Generalized Algorithm for Nutrient, Growth, Stoichiometric and Thermodynamic Analysis (GANGSTA) to posit the mechanisms by which dynamic interactions among elemental cycles emerge.
- Metabolism
- Modeling
- Mesocosm
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Presenters/Authors
Elizabeth Mohr
(), Montana State University, elizabethjmohr@gmail.com;
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Ann Marie Reinhold
(), Montana State University, Montana Institute on Ecosystems, reinhold@montana.edu;
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Robert Payn
(), Montana State University, Montana Institute on Ecosystems, rpayn@montana.edu;
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Robert O. Hall
(), Flathead Lake Biological Station, University of Montana, bob.hall@flbs.umt.edu;
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Joanna Blaszczak
(), Global Water Center and Department of Natural Resources and Environmental Science, University of Nevada, Reno, jblaszczak@unr.edu;
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Geoffrey Poole
(), Montana State University, Montana Institute on Ecosystems, gpoole@montana.edu ;
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