2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 2:00 PM - 3:30 PM | DELIMITING CRYPTIC WINTER-EMERGING CHIRONOMIDAE (INSECTA: DIPTERA) OF THE SOUTHEASTERN MINNESOTA DRIFTLESS AREA UTILIZING MOLECULAR TECHNIQUES | Virtual Platform
DELIMITING CRYPTIC WINTER-EMERGING CHIRONOMIDAE (INSECTA: DIPTERA) OF THE SOUTHEASTERN MINNESOTA DRIFTLESS AREA UTILIZING MOLECULAR TECHNIQUES
Chironomidae that emerge in temperate regions during winter are less frequently studied as compared to summer-emerging counterparts. Two commonly encountered winter-emerging species, Diamesa nivoriunda Fitch and Diamesa mendotae Muttkowski have not been molecularly analyzed. Diamesa nivoriunda is widespread and well-defined morphologically. Diamesa mendotae is more restricted in distribution but more variable morphologically. Both species were collected in the southeastern Minnesota Driftless Area along four groundwater dominated trout streams during winter 2019-2020: Pine Creek, Clear Creek, Miller Creek, and the Vermillion River. Sixty-seven of 302 adults collected in December 2019 were randomly chosen for genetic analysis. Mitochondrial and nuclear DNA were extracted and barcode regions COI (154 bp), EF-1a (591 bp), and ITS (1044 bp) were amplified and Sanger sequenced. Multiple distinct sequences of Diamesa nivoriunda (12) and Diamesa mendotae (189) were differentiated across streams: Pine Creek (66), Clear Creek (54), Miller Creek (45), and Vermillion River (36). The number of unique sequences as a function of barcode regions were: 8 for COI, 3 for EF-1a, and 15 for ITS. The degree of differentiation of sequences suggest the presence of cryptic species within both morphologically defined species.
- Climate variability
- Global change
- Stream
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Presenters/Authors
Tessa Durnin
(), University of Minnesota, Twin Cities, durni018@umn.edu;
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Debora Pires Paula
(), Embrapa, debora.pires@embrapa.br ;
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Leonard C. Ferrington, Jr.
(), University of Minnesota, ferri016@umn.edu;
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