2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 8:30 AM - 10:30 AM | A Novel Method of Monitoring the Health of our Global Freshwater Supply using DNA Barcoding | Virtual Platform
A Novel Method of Monitoring the Health of our Global Freshwater Supply using DNA Barcoding
It is forecast that 66% of our population will experience water scarcity within a decade, leaving us more dependent on surface water for drinking. This requires more filtration infrastructure, and monitoring of surface water sources. Current methods rely on expensive and technically challenging manual identification of biological samples. Macroinvertebrates spend their larval lives within a small area of water, showing cumulative effects of habitat alteration and pollutants that chemical testing and field sensors do not. Molecular methods enhance biomonitoring programs where complex taxonomy makes manual morphological identification difficult. This project explores deoxyribonucleic acid (DNA) barcoding, to measure waterway health, particularly with the most widespread macroinvertebrate families. A statistical sampling plan was designed that represents variation in geological, ecological, and land use factors. Four methods of isolation and amplification were compared. Statistical analysis shows DNA Barcoding results in more accurate and precise waterway health data, adding significant value for monitoring scarce water resources. The learnings from these data were applied to build a microbiology capability at a nonprofit scientific water study institute. This method can be applied at low cost to enhance citizen science water monitoring programs.
- Management
- Interdisciplinary
- Genomics
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Presenters/Authors
Sonja Michaluk
(), Carnegie Mellon University , sonja.michaluk@gmail.com;
ASHA DISCLOSURE:
Financial -
Nonfinancial -