2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/27/2021  |   2:00 PM - 3:30 PM   |  A NOVEL THIN-FILM METHOD TO IMPROVE ACCURACY OF FLUORESCENCE-BASED PHOTOPHYSIOLOGICAL ESTIMATES IN PERIPHYTIC BIOFILMS: A NUMERICAL AND EMPIRICAL EVALUATION   |  Virtual Platform

A NOVEL THIN-FILM METHOD TO IMPROVE ACCURACY OF FLUORESCENCE-BASED PHOTOPHYSIOLOGICAL ESTIMATES IN PERIPHYTIC BIOFILMS: A NUMERICAL AND EMPIRICAL EVALUATION

Recent studies suggest that photophysiological parameters for intact substrates with depth (e.g. periphytic biofilms, microphytobenthos) are overestimated by pulse-amplitude modulated (PAM) fluorometry. This overestimation stems from depth-integration effects resulting from activation of deeper photosynthesizing layers by an attenuated light signal. Here we propose a novel thin-film technique in which fluorescence is measured on a representative subsample spread evenly on a microscope slide. We compare bias and precision in fluorescence-based estimates using the novel thin-film method and conventional intact biofilms. In line with previous studies, numerical simulations of periphytic biofilms demonstrated substantial reduction in bias with the thin-film method. Key parameters were overestimated for intact biofilms, with relative errors up to 145%, compared to 52% on thin films. Paired empirical observations likewise demonstrated that estimates for intact biofilms were consistently higher (up to 248%, p < 0.001) than for thin films. While bias is reduced, poor subsampling for the thin film may diminish precision. Nonetheless, empirically-based estimates of precision for replicate thin-film and intact measurements demonstrated slight improvement in precision for the thin-film method (e.g. the coefficient of variation for the maximum electron transport rate was reduced 30%, p = 0.009).

  • Microbial ecology
  • Models
  • Stream

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Presenters/Authors

Katie Hossler (), Wright State University, katie.hossler@wright.edu;


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Leon Katona (), Wright State University , katona.2@wright.edu;


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Yvonne Vadeboncoeur (), Wright State University, yvonne.vadeboncoeur@wright.edu;


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