2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 2:00 PM - 3:30 PM | PREVALENCE OF THE MICROSPORIDIAN PARASITE THELOHANIA CONTEJEANI IN CRAYFISH ACROSS FIVE OHIO WATERSHEDS | Virtual Platform
PREVALENCE OF THE MICROSPORIDIAN PARASITE THELOHANIA CONTEJEANI IN CRAYFISH ACROSS FIVE OHIO WATERSHEDS
Thelohania contejeani is a parasitic microsporidian that causes porcelain disease (PD) in crayfish. This study utilized molecular methods to survey for T. contejeani across five Ohio river drainages. Crayfish were collected in 2019 (N=31) and in 2020 (N=97) and tissue samples were stored in 95% ethanol for subsequent genetic analyses. Total genomic DNA (gDNA) was extracted from samples using a Qiagen DNeasy Blood and Tissue Kit®. The ITS1 gene of microsporidia was then amplified via polymerase chain reaction (PCR). Successful amplifications served as template in PCR reamplifications using primers specific for T. contejeani. In 2019, one crayfish (3%) out of 12 sampled from the Muskingum River was positive for T. contejeani. In 2020, T. contejeani was detected in 16.7% of the crayfish sampled from the Maumee River (N=24) and 17.6% from the Great Miami River (N=17), however PD-infected crayfish were not found in the Muskingum (N=26), Scioto (N=10), nor Little Miami Rivers (N=20). DNA barcoding will be utilized to identify host species as crayfish may be differentially susceptible to PD, information that could be utilized for prioritizing conservation efforts.
- Contaminants
- Microbial ecology
- Conservation
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Presenters/Authors
Kourtney N. Craft
(), Ohio Northern University, k-craft@onu.edu;
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Steven C. Churchin
(), Ohio Northern University, s-churchin@onu.edu;
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Tyler A. Koncsol
(), Ohio Northern University, t-koncsol@onu.edu;
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Leslie Riley
(), Ohio Northern University, l-riley.1@onu.edu;
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Robert Verb
(), Ohio Northern University, r-verb@onu.edu;
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Emily M. Imhoff
(), Cincinnati Museum Center, eimho@cincymuseum.org;
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Katherine L. Krynak
(), Ohio Northern University, k-krynak@onu.edu;
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Devon Jackson
(), Ohio Northern University, Department of Biological & Allied Health Sciences, d-jackson.5@onu.edu;
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