2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
6/06/2017 | 3:15 PM - 3:30 PM | THE USE OF ENVIRONMENTAL DNA FOR THE DETECTION OF A NARROWLY ENDEMIC CRAYFISH IN A HIGH-DISCHARGE, LOTIC ECOSYSTEM | 305A
THE USE OF ENVIRONMENTAL DNA FOR THE DETECTION OF A NARROWLY ENDEMIC CRAYFISH IN A HIGH-DISCHARGE, LOTIC ECOSYSTEM
Narrowly endemic crayfishes often experience elevated risk for extinction given various biological, chemical, and physical threats facing their ecosystems. Orconectes eupunctus is a narrowly endemic crayfish inhabiting large streams in the Eleven Point River drainage in Arkansas and Missouri. We conducted a drainage-wide survey for O. eupunctus using conventional kick seine surveillance methods to assess the species’ distribution and potential abiotic habitat associations. Additionally, we employed environmental DNA (eDNA) surveillance methods to compliment species presence data obtained via conventional surveillance methods. Environmental DNA surveillance methods are potentially more sensitive to species presence given the ability of modern molecular analysis techniques to detect trace amounts of DNA from environmental samples, which may increase our ability to detect species in cases where conventional species surveillance methods do not. Orconectes eupunctus was detected at 38% of sites using conventional surveillance methods and showed habitat associations with mean substrate size, current velocity, and depth. Results from eDNA surveillance methods generally agreed with results from conventional surveillance methods, thus demonstrating that eDNA can reliably detect the presence of benthic organisms in high-discharge lotic ecosystems.
- C03 Invertebrates
- C30 Molecular Ecology
- S12 Challenges, insights, and opportunities in crayfish conservation and ecology
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Presenters/Authors
Christopher Rice
(), University of Illinois, cjrice3@illinois.edu;
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Eric Larson
(), University of Illinois, erlarson@illinois.edu;
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Christopher A. Taylor
(), Illinois Natural History Survey, cataylor@illinois.edu;
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