2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/21/2018 | 2:00 PM - 2:15 PM | A GUT FEELING: THE EFFECTS OF LEAF LITTER TYPE ON THE ACTIVE MICROBIOME OF A SHREDDING CADDISFLY | 330 A

A GUT FEELING: THE EFFECTS OF LEAF LITTER TYPE ON THE ACTIVE MICROBIOME OF A SHREDDING CADDISFLY

Insect gut microbes facilitate the digestion of plant material supporting the idea that the gut microbiome is a crucial component in freshwater detritivores. We used quantitative stable isotope probing (qSIP), a molecular method which differentiates actively growing microbes from inactive microbes to investigate the gut microbiome of a detritivore shredder, Hesperophlax magnus. qSIP allowed us to tease apart “transient microbes” (i.e., those passing through the gut) from “facilitative microbes” (i.e., gut residents). We conducted a laboratory mesocosm experiment in which two different leaf species were conditioned for ten days, colonizing with their respective microbial communities. Shredders then fed on the conditioned leaves for four days and afterwards, were placed in ¹⁸O labeled water for 2-5 days. Growing microbial cells in the gut incorporated the ¹⁸O label. By measuring this isotopic label and sequencing the newly labeled DNA of gut microbes, we quantified growth, thereby distinguishing organisms that were active in the gut from inactive microbes that were ingested. In this talk we demonstrate how qSIP can be used to estimate taxon-specific growth rates of bacteria in the microbiome of shredding insects.

Invertebrate
New Methods
Isotope

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Presenters/Authors

Rebecca Fritz (), Northern Arizona University, rjf227@nau.edu;