2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/22/2018 | 3:15 PM - 3:30 PM | MORPHOLOGICAL AND DNA METHODOLOGIES IN ASSESSING BENTHIC MACROINVERTEBRATE COMMUNITIES | 420 B
MORPHOLOGICAL AND DNA METHODOLOGIES IN ASSESSING BENTHIC MACROINVERTEBRATE COMMUNITIES
In 2015, we collected aquatic macroinvertebrates from 13 national parks in the National Capital Region Network. Historically, the National Park Service has sampled 11 of these sites and analyzed these data by subsampling 100 individuals. The first objective of this study was to determine the efficacy of only examining subsamples. Thus, we identified all organisms in the sample and compared this to 50 permutations of randomly selecting 100, 200 and 300 organism subsamples. The Brillouin diversity index of the entire sample was higher than that calculated on the computer-generated subsamples 75% of the time, illustrating that biodiversity is masked using traditional morphological protocols. Therefore, we explored the use of eDNA to evaluate samples and improve macroinvertebrate bioassessments. The second objective of this study was to examine the use of COI and 16S as markers for metabarcoding of aquatic macroinvertebrates. Ultimately, we made a large contribution to the effort of building a robust 16S rDNA barcode database for aquatic macroinvertebrate metabarcoding through the generation and curation of 74 unique sequences.
- Invertebrate
- Biodiversity
- Genetics
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Presenters/Authors
Sara Mueller
(), The Pennsylvania State University, sjm5467@psu.edu;
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Aaron Aunins
(), U.S. Geological Survey, aaunins@usgs.gov;
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Jay Stauffer
(), The Pennsylvania State University, vc5@psu.edu;
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