2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 2:00 PM - 3:30 PM | USING COMMUNITY METABARCODING OF ZOOPLANKTON TO ASSESS TRENDS ACROSS NEON LAKE SITES | Virtual Platform
USING COMMUNITY METABARCODING OF ZOOPLANKTON TO ASSESS TRENDS ACROSS NEON LAKE SITES
The National Ecological Observatory Network (NEON) is research platform designed to assess the effects of ecological change on ecosystems across North America. NEON’s aquatic program includes of a suite of instrument and observational data collected at 34 sites: 24 wadeable streams, 7 lakes, and 3 large rivers ranging from Puerto Rico to Alaska. Zooplankton samples are collected three times per year (spring, summer, and fall) at all 7 NEON lake sites. Zooplankton samples are collected for analysis using vertical tow nets or Schindler traps during mid-summer, with additional samples collected in spring and fall to be archived in bulk. Metabarcode samples are homogenized prior to DNA extraction, and two fragments of the COI gene are PCR-amplified and Illumina-sequenced. Data are processed through a bioinformatics pipeline. Both processed bioinformatics data as well as raw sequence data are publicly available and hosted on the NEON data portal. DNA extracts and bulk samples from spring and fall sampling are stored at the NEON bioarchive facility for further use by the community. These data and archived samples are a novel resource that will enable analyses over large spatial and temporal scales.
- Climate change
- Big data
- Monitoring
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Presenters/Authors
Stephanie Parker
(), NEON/Battelle, sparker@battelleecology.org;
ASHA DISCLOSURE:
Financial -
Nonfinancial -