2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/23/2019 | 2:00 PM - 2:15 PM | PERSISTENCE AND ACCUMULATION OF ENVIRONMENTAL DNA FROM AN ENDANGERED DRAGONFLY: PRELIMINARY RESULTS | 250 DE
PERSISTENCE AND ACCUMULATION OF ENVIRONMENTAL DNA FROM AN ENDANGERED DRAGONFLY: PRELIMINARY RESULTS
Detection of environmental DNA (eDNA) is becoming a commonly used survey method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, its application for the detection of invertebrates is not as well developed. Developing eDNA detection protocols are especially important for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by the training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is the evaluation of the dynamics of eDNA concentration. We used quantitative PCR to examine the persistence and accumulation of eDNA from captive S. hineana larvae in experimental mesocosms at water temperatures (5 and 16 Celsius) that reflect seasonal variation in their natural habitat. There was detectable variation in the accumulation and persistence of eDNA at different temperatures. Differences in the accumulation and persistence of eDNA at these temperatures reflect not only differences in longevity of eDNA, but also seasonal differences in behavior of S. hineana impacting their rates of DNA loss.
- Invertebrate
- Wetland
- Population
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Presenters/Authors
Kristie Schmidt
(), University of South Dakota, Kristie.Schmidt@coyotes.usd.edu;
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Sarah Maestas
(), University of South Dakota, sarahmays12@gmail.com;
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Daniel Soluk
(), University of South Dakota, daniel.soluk@usd.edu ;
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Hugh Britten
(), University of South Dakota, hugh.britten@usd.edu;
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