2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
6/05/2017 | 2:00 PM - 2:15 PM | IMPACTS OF TRICLOSAN EXPOSURE ON ANTIMICROBIAL RESISTANCE IN STREAM BACTERIAL ISOLATES | 305B
IMPACTS OF TRICLOSAN EXPOSURE ON ANTIMICROBIAL RESISTANCE IN STREAM BACTERIAL ISOLATES
Antimicrobial resistance (AMR) is a major global health challenge. Multidrug-resistance compromises efficacy of antibiotics and hinders infectious disease treatment. Triclosan brings numerous health risks including multidrug-resistance and is commonly found in human and environmental samples. Acquired resistance to triclosan and associated multidrug-resistance has occurred with clinical isolates. Similar patterns in streams or wastewater treatment plants threaten human health. The study goal is to evaluate stream bacterial responses to environmentally-relevant triclosan exposure. Triclosan exposure was predicted to impact susceptibility to triclosan and other antibiotics. Wastewater-associated and forested-stream periphyton were hypothesized to exhibit different overall levels of susceptibility. Triclosan resistance was hypothesized to associate with triclosan exposure and with resistance to other antibiotics. Periphyton underwent 10 microgram/L triclosan exposures in microcosms. Bacterial isolates from microcosms were assayed for AMR through broth microdilution and identified through 16S rRNA sequencing. Exposed and unexposed wastewater-associated isolates showed similar triclosan susceptibility, while exposed forested-stream isolates showed lower triclosan susceptibility compared to those unexposed. Most isolates exhibiting triclosan resistance also show resistance to carbenicillin, chloramphenicol, erythromycin, and trimethoprim. Results of this study indicate that triclosan in streams may alter AMR, possibly adding to the problem of multidrug-resistance.
- C04 Microbial Ecology
- C08 Urban Ecology
- C13 Ecotoxicology
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Presenters/Authors
Kirsten Trowbridge
(), University of North Carolina at Greensboro, ketrowbr@uncg.edu;
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