2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

5/20/2019 | 10:15 AM - 10:30 AM | MICROBIAL COMMUNITY DIVERSITY IN URBAN STREAMS RECEIVING WASTEWATER EFFLUENT | 253 AB

MICROBIAL COMMUNITY DIVERSITY IN URBAN STREAMS RECEIVING WASTEWATER EFFLUENT

Microbial communities are critical components of freshwater ecosystems that vary along spatiotemporal gradients and drive material cycling and energy flow. In urban streams, multiple stressors can affect these communities and influence ecosystem function. We quantified microbial abundance and diversity from three habitats (surface water, sediment, riparian soil) in two creeks in Charlotte, N.C. that are receiving treated wastewater. We collected samples from two sites upstream and downstream of WWTPs for both creeks. We used Illumina 16S sequencing to assay the microbial community at each site across four time points from late winter to mid-summer of 2016. As expected, water, sediment, and soil microbial communities were separate and unique at all taxonomic levels from phylum to genus. There were slight differences across time for each habitat and over half of the taxa were similar between upstream and downstream sites. In a companion project, we quantified an increase in antibiotic concentrations downstream of the WWTP and a slight suppression of functional pathways in the surface water microbial community. Further studies should investigate the impact of chronic, low concentrations antibiotics on both surface water and sediment microbial community function.

Urban
Metagenomics
Bacteria

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Presenters/Authors

Sandra Clinton, PhD (), University of North Carolina at Charlotte, sclinto1@uncc.edu;