2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/25/2021 | 8:30 AM - 10:30 AM | THE EFFECTS OF SALINITY AND N:P ON CYANOTOXINS IN A N-FIXING AND NON-N-FIXING CYANOBACTERIA | Virtual Platform

THE EFFECTS OF SALINITY AND N:P ON CYANOTOXINS IN A N-FIXING AND NON-N-FIXING CYANOBACTERIA

The effect of increasing salinity on the growth and toxin production/leakage of harmful algal blooms (HABs) has not been widely studied. Coastal regions are beginning to experience how encroaching salt water conditions, due to rising sea levels caused by climate change, are affecting freshwater phytoplankton, with HABs being a key concern due to their ability to produce cyanotoxins. The production of nitrogen (N)-rich cyanotoxins has been shown to increase with N availability to blooms, but N is often the limiting nutrient in coastal marine ecosystems. To test how an increase in salinity will affect both microcystin-LR and cylindrospermopsin concentration both over time and under differing N availabilities, we grew Microcystis and Aphanizomenon in batch cultures for approximately a month under low (4) and high (50) N:P ratio environments. We then simulated flushing events to ocean water (OW) environments by spiking cultures with differing amounts of artificial OW (0-30%) after 21 days of growth. After, we monitored growth, carbon (C) and N stoichiometry, and toxin dynamics for 10 days. Our results suggest that the N:P of growth conditions determines how both time and salinity concentration will influence cyanotoxin production/leakage.

Stoichiometry
Nutrients
Stressor

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Presenters/Authors

Felicia Osburn (), Baylor University, felicia_osburn1@baylor.edu;