2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/21/2019  |   2:15 PM - 2:30 PM   |  THE MICROBIOME OF STREAM MACROINVERTEBRATES: CONNECTING COMMUNITY COMPOSITION TO ECOSYSTEM FUNCTION   |  150 G

THE MICROBIOME OF STREAM MACROINVERTEBRATES: CONNECTING COMMUNITY COMPOSITION TO ECOSYSTEM FUNCTION

Stream microorganisms perform critical biogeochemical functions including denitrification (an important process that removes bioavailable N). Likewise, macroinvertebrates are ecologically important and potentially represent a novel habitat for microorganisms. We demonstrated that stream macroinvertebrates have a diverse microbiome and that composition and diversity vary among hosts. Macroinvertebrate functional feeding groups and stream (anthropogenically impacted versus unimpacted) determined gut bacterial diversity; 79 phylotypes differed significantly among macroinvertebrate types. These microbiomes include microorganisms that have functional genes capable of performing a variety of aspects of the N cycle, including denitrification. Capabilities of the macroinvertebrate microbiome were put in context by comparing denitrification rates among macroinvertebrates and sediment and water from a fourth order stream (OH, USA). Macroinvertebrates sampled sufficiently were from the families Cambaridae, Athericidae, Baetiscidae, and Hydropsychidae. Denitrification rates were about 4 times higher in macroinvertebrates than in sediments. Denitrification rates ranged from 0.27µLN2O-N g wet weight (Athericidae) to 2.24µLN2O-N g wet weight (Cambaridae) and differed among families. Ongoing work will relate functional genes (narG, nirS, and nosZ ) to denitrification rates. In conclusion, stream macroinvertebrates harbor diverse communities that differ among taxa and appear to be important to ecosystem processes.

  • Biogeochemistry
  • Nitrogen
  • Bacteria

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Presenters/Authors

Sohini Bhattacharyya (), Kent State University, sbhatta6@kent.edu;


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Paul Ayayee (), University of Wyoming, akwettey@gmail.com;


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Laura Leff (), Kent State University, lleff@kent.edu;


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