2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
6/08/2017 | 11:30 AM - 11:45 AM | EXPLORING FRESHWATER MICROBIOMES USING QUANTITATIVE STABLE ISOTOPE PROBING | 305B
EXPLORING FRESHWATER MICROBIOMES USING QUANTITATIVE STABLE ISOTOPE PROBING
Freshwater microbes associated with leaf litter decomposition serve as a food resource for aquatic insects. Insects also have microbial communities in their guts to assist digestion. Quantitative stable isotope probing (qSIP) is a novel molecular tool to differentiate active from inactive microbes. This technique uses, ‘heavy water,’ H218O to isotopically label microbial taxa and estimate their growth rates. This experiment addresses three questions: Does the activity rate of individual microbial taxa differ across leaf types during decomposition? How does the presence of shredders affect the microbial assemblage and growth rates of individual taxa? Do the microbes that are active in the guts of insects reflect the microbes found on decomposing leaves? Results indicate that microbial communities differ across leaf types and during decomposition. Growth rates of microbes are not correlated with their abundances suggesting that taxa in low abundance might be important in organic matter processing. We will discuss a model for viewing microbes with respect to ecological roles often used to describe macroscopic organisms.
- C25 Food Webs
- C11 Community Ecology
- C04 Microbial Ecology
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Presenters/Authors
Rebecca Fritz
(), Northern Arizona University, rjf227@nau.edu;
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Michaela Hayer
(), Northern Arizona University, michaela.hayer@nau.edu;
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Rebecca Mau
(), Northern Arizona University, rlm284@nau.edu;
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Benjamin Koch
(), Northern Arizona University, ben.koch@nau.edu;
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Alexa Schuettenberg
(), Northern Arizona University, aas322@nau.edu;
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Egbert Schwartz
(), Northern Arizona University, egbert.schwartz@nau.edu;
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Bruce Hungate
(), Northern Arizona University, bruce.hungate@nau.edu;
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Jane Marks
(), Northern Arizona University, jane.marks@nau.edu;
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