2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

5/22/2018 | 11:15 AM - 11:30 AM | VARIATION IN PHOSPHORUS USE AMONG RECOMBINANT DAPHNIA GENOTYPES | 321

VARIATION IN PHOSPHORUS USE AMONG RECOMBINANT DAPHNIA GENOTYPES

Resurrecting diapausing (resting) eggs of the aquatic keystone herbivore, Daphnia, preserved in lake sediments for centuries has revealed striking shifts in nutrient use physiology that are correlated with nutrient enrichment (i.e., cultural eutrophication). As such, this system allows us to address key issues in ecological genomics with a unique temporal approach that is not possible with more common space-for-time substitutions (i.e., comparing populations to infer evolutionary trajectories). Previous work illuminated transcriptome-wide variation underlying such major changes in phosphorus kinetics between ancient and modern genotypes. Here, we use quantitative trait loci (QTL) mapping to identify genomic regions underlying P-use. An F2 mapping population has been constructed by crossing a ~5-yr-old (dam) clone with a ~50-yr-old (sire) clone that differ in P-use. F2 genotypes exhibit substantial physiological variation in P-use. These genotypes have been sequenced to identify loci underlying differences in P-use. By identifying sections of the genome that enable Daphnia to be P sinks in lakes we should be able to generate predictions about the evolution of Daphnia populations in response to eutrophication, and concomitant changes in the key ecological functions they perform.

Zooplankton
Genetics
Stoichiometry

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Presenters/Authors

Ryan Sherman (), Oklahoma State University, ryan.sherman@okstate.edu;