2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/22/2019 | 9:45 AM - 10:00 AM | BENTHIC MACROINVERTEBRATE COMMUNITIES ACROSS THE NEON OBSERVATORY: USING METABARCODE AND MORPHOLOGICAL TAXONOMY DATA TO ASSESS TRENDS | 254 B
BENTHIC MACROINVERTEBRATE COMMUNITIES ACROSS THE NEON OBSERVATORY: USING METABARCODE AND MORPHOLOGICAL TAXONOMY DATA TO ASSESS TRENDS
The National Ecological Observatory Network (NEON) is research platform designed to assess the effects of ecological change on ecosystems across North America. NEON’s aquatic program includes of a suite of instrument and observational data collected at 34 sites: 24 wadeable streams, 7 lakes, and 3 large rivers ranging from Puerto Rico to Alaska. Benthic macroinvertebrate samples are collected three times per year (spring, summer, and fall) at all NEON aquatic sites. Benthic macroinvertebrate samples are collected using quantitative methods for morphological taxonomic analysis, while a second set of samples are collected at the same time and location for metabarcode analysis. Morphological taxonomy samples are processed and identified to the lowest practical taxonomic level. Metabarcode samples are homogenized prior to DNA extraction, and two fragments of the COI gene are PCR-amplified and Illumina sequenced. NEON morphological taxonomy data are publically available and hosted on the NEON data portal, while sequence data are hosted on MG-RAST. DNA extracts are stored at the NEON bioarchive facility for further use by the community. These data and archived samples are a novel resource that will enable analyses over large spatial and temporal scales.
- Genetics
- Monitoring
- Assessment
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Presenters/Authors
Stephanie Parker
(), NEON/Battelle, sparker@battelleecology.org;
ASHA DISCLOSURE:
Financial -
Nonfinancial -