2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/22/2018 | 12:00 PM - 12:15 PM | USING EDNA TO ELUCIDATE DISTRIBUTION OF FISH SPECIES IN A COMPLEX RIVER SYSTEM | 420 B

USING EDNA TO ELUCIDATE DISTRIBUTION OF FISH SPECIES IN A COMPLEX RIVER SYSTEM

Environmental DNA (eDNA) analysis is a noninvasive genetic tool that can improve efficiency and reduce costs associated with species detection in aquatic systems. eDNA methods are widely used to assess presence/absence of target species and have recently demonstrated potential for estimating biodiversity in controlled environments. We applied eDNA methods to estimate distribution of fish species in a large and complex system. The St. Louis River Estuary provides a suitable study area because fish diversity and species distribution is well documented, thanks to past and ongoing fishery assessments and invasive species early detection monitoring. We collected water samples from 240 randomly selected sites extending over 30 river km in June and October. At 30 sites, an additional water sample was collected for an inter-lab comparison. Composition and spatial distribution patterns derived from eDNA data are being compared to traditional adult and larval fish survey data to understand the different perspectives they give. Results from our study provide insight into the efficacy of eDNA methods for estimating fish distribution in large, complex aquatic systems and begin to inform us about how these methods can be operationalized for use by management agencies.

Metagenomics
Distribution
Bioassessment

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Presenters/Authors

Chelsea Hatzenbuhler (), USEPA/NHEERL/MED,ORISE, Duluth, MN, hatzenbuhler.chelsea@epa.gov ;