2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/26/2021 | 8:30 AM - 10:30 AM | FRESHWATER BIODIVERSITY AND SPECIES IDENTIFICATION USING MICROSCOPY, DNA BARCODING AND METAGENOMICS APPROACHES: A COURSE-BASED UNDERGRADUATE RESEARCH EXPERIENCE | Virtual Platform
FRESHWATER BIODIVERSITY AND SPECIES IDENTIFICATION USING MICROSCOPY, DNA BARCODING AND METAGENOMICS APPROACHES: A COURSE-BASED UNDERGRADUATE RESEARCH EXPERIENCE
An inquiry-based, learner-centered approach to teaching allows students to grow both in basic STEM knowledge and as researchers. We developed a semester-long project investigating local freshwater microbial diversity through microscopy, metabarcoding, and targeted sequencing as part of the junior-level biology curriculum at a small liberal arts college during the Spring 2021 semester. In the first half of the semester, students gained field experience while taking water samples and abiotic measurements from local freshwater sources, lab experience in microscopy, filtering the samples, extracting the DNA, running PCRs, and preparing the samples for sequencing, and exposure to bioinformatic techniques through the Mothur 16S rRNA pipeline implemented on Galaxy servers. Analyzing biotic diversity measures alongside water chemistry measurements allows students to connect characteristics of the biotic and abiotic environments. In the second half of the semester students developed their own projects using the microscopy, field, and/or bioinformatic techniques explored in the first half. This approach provides students with the tools and knowledge to develop their own projects and the space to explore their interests as related to the course, building independence and sparking enthusiasm for continued exploration in STEM.
- Microbial ecology
- Data science
- Genomics
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Presenters/Authors
Amanda Glazier
(), Haverford College, aglazier@haverford.edu;
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Halie Rando
(), Haverford College, hrando@haverford.edu;
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Nile Bayard
(), Haverford College, nbayard@haverford.edu ;
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Maya Casey
(), Haverford College, mtcasey@haverford.edu ;
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Genevieve Dallmeyer-Drennen
(), Haverford College, gdallmeyer@haverford.edu;
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Maddie Figueredo
(), Haverford College, mfigueredo@haverford.edu;
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Naomi Fukuda
(), Haverford College, nfukuda@haverford.edu ;
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Eleanor Greene
(), Haverford College, egreene2@haverford.edu;
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Emma Iacobucci
(), Haverford College, eiacobucci@haverford.edu>;
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Dina Kosyagin
(), Haverford College, dkosyagin@haverford.edu;
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Benjamin Kwon
(), Haverford College, ykwon@haverford.edu>;
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Annette Lee
(), Haverford College, clee9@haverford.edu;
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Andre Martin
(), Haverford College, almartin@haverford.edu ;
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Jacob Melnick
(), Haverford College, jmelnick@haverford.edu;
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Junior Nguyen
(), Haverford College, jnguyen4@haverford.edu;
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Ryan O'Donnell
(), Haverford College, rodonnell@haverford.edu;
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Riley Outen
(), Haverford College, routen@haverford.edu;
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Julie Rebh
(), Haverford College, jrebh@haverford.edu;
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Giancarlo Rendon
(), Haverford College, grendon@haverford.edu ;
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Taylor Seid
(), Haverford College, tseid@haverford.edu;
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Roy Simamora
(), Haverford College, rsimamora@haverford.edu ;
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Carmiya Solomon
(), Haverford College, casolomon@haverford.edu ;
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William Vostrejs
(), Haverford College, wvostrejs@haverford.edu ;
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Katherine Wass
(), Haverford College, kwass@haverford.edu;
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Rachel White
(), Haverford College, rswhite@haverford.edu ;
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Han Yang
(), Haverford College, hyang3@haverford.edu;
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Maria Yea
(), Haverford College, iyeaseong@haverford.edu;
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Amra Zegeye
(), Haverford College, aezegeye@haverford.edu;
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Yifan Zhang
(), Haverford College, yzhang12@haverford.edu;
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Rachel Hoang
(), Haverford College, rhoang@haverford.edu;
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