2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
6/05/2017 | 2:45 PM - 3:00 PM | SUB-LETHAL METABOLIC RESPONSES TO CONTAMINANT MIXTURE TOXICITY IN DAPHNIA MAGNA | 305B
SUB-LETHAL METABOLIC RESPONSES TO CONTAMINANT MIXTURE TOXICITY IN DAPHNIA MAGNA
Anthropogenic factors are increasing the presence of contaminants in freshwater ecosystems, entering waterways through wastewater effluents and urban and/or agricultural runoff. While the concentration of contaminants in surface waters is very low, their occurrence within a complex mixture can cause profound repercussions on aquatic organisms. Here we examined Daphnia magna metabolic responses after sub-lethal (10% of the LC50) acute exposure to propranolol (PRO), carbamazepine (CBZ), and perfluorooctanesulfonic acid (PFOS), and the binary (PRO-CBZ, PRO-PFOS, CBZ-PFOS) and tertiary mixtures (CBZ-PRO-PFOS). The metabolome was measured using 1H nuclear magnetic resonance and characterized using principal component analysis, regression analysis and changes in metabolite concentrations relative to the unexposed group. The averaged PCA scores plots revealed CBZ-PFOS and CBZ-PRO-PFOS exposures were significantly different from the control. These two mixtures also displayed the most pronounced changes in identified metabolites. Overall, our results indicate the severity of toxicity depends on the composition of the contaminant mixture, with some mixtures displaying heightened toxicity versus others.
- C03 Invertebrates
- C17 Bioassessment
- C13 Ecotoxicology
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Presenters/Authors
Nicole Wagner
(), University of Toronto, nicolegouldingwagner@gmail.com;
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André Simpson
(), University of Toronto, andre.simpson@utoronto.ca;
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Myrna Simpson
(), University of Toronto, myrna.simpson@utoronto.ca;
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