2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/27/2021 | 2:00 PM - 3:30 PM | Exploring the Role of Biofilm Colonization on the Transport and Fate of Environmental DNA (eDNA) | Virtual Platform

Exploring the Role of Biofilm Colonization on the Transport and Fate of Environmental DNA (eDNA)

The use of environmental DNA (eDNA) analysis offers insight into the distribution of invasive and endangered aquatic species without intensive traditional sampling. However, our understanding of eDNA fate and transport in flowing waters is limited, which constrains predictions about the location and density of target organisms. We used sequential filtration to investigate the size distribution of eDNA from Common Carp and found that the majority were in the 1.0 micrometer size category, although both larger and smaller particles were present. We also used short-term additions of carp eDNA to experimental streams to quantify the effect of biofilm colonization on eDNA removal from the water column. We hypothesized that there would be shorter transport lengths (Sw) in streams with the highest biofilm colonization. After refinement of molecular extraction techniques, our preliminary analysis of the field releases showed that under low biofilm (i.e., shaded) conditions, Sw was measurable (17 m), and additional analyses are ongoing. Improved understanding of eDNA particle size distribution in relation to biofilm conditions will enhance our ability to predict eDNA transport in streams and rivers and advance the use of eDNA as a tool for monitoring and management.

Management
Vertebrates
Stream

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Presenters/Authors

Elise Snyder (), The University of Notre Dame, esnyder4@nd.edu;