2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/22/2018 | 2:45 PM - 3:00 PM | THE COLLECTIVE EFFECTS OF WASTEWATER EFFLUENT AND INDIVIDUAL EFFECTS OF CAFFEINE ON STREAM BIOFILMS | 420 A
THE COLLECTIVE EFFECTS OF WASTEWATER EFFLUENT AND INDIVIDUAL EFFECTS OF CAFFEINE ON STREAM BIOFILMS
Background/Methods:
The introduction of wastewater effluent to receiving streams brings possible effects on species interactions, food webs and aquatic-terrestrial linkages. With this study, we focused on these effects on biofilm at the base of the stream food web. We also aim to tease out the effects of caffeine on stream biofilm, as it is commonly associated with effluent.
We collected samples of effluent from 12 wastewater facilities in Northwest Ohio, as well as stream water upstream and downstream of each outfall location in receiving streams. These water samples were tested for the concentrations of various nutrients and of caffeine (ELISA). Biofilm was collected at these same locations and analyzed for microbial community composition (16s and 18s sequencing) and nutritional values (C:N:P).
Conclusions/Results:
This study resulted in significant differences in DRP, NO2NO3, and caffeine concentrations among upstream, outfall, and downstream sampling locations. There were also significant differences in biofilm eukaryotic community composition and C:P values. This study was invaluable in formulating multiple hypotheses regarding the collective effects of effluent as well as justifying a follow-up study on the effects of caffeine in the field via chemical diffusing substrata.
- Community
- Food Webs
- Water Quality
Presentation:
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Presenters/Authors
Melanie Marshall
(), Bowling Green State University, melaniemarshall14@gmail.com;
ASHA DISCLOSURE:
Financial -
Nonfinancial -
Kevin McCluney
(), Bowling Green State University, kmcclun@bgsu.edu;
ASHA DISCLOSURE:
Financial -
Nonfinancial -