2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/23/2019 | 9:45 AM - 10:00 AM | A 10 MINUTE GUIDE TO DNA METABARCODING MACROINVERTEBRATES | 150 DEF
A 10 MINUTE GUIDE TO DNA METABARCODING MACROINVERTEBRATES
DNA metabarcoding is an excellent tool for rapid assessment of biodiversity. This presentation will outline a workflow from start to finish, allowing the processing of 80+ macroinvertebrate bulk samples within 10 work days, including bioinformatics. Two DNA extraction methods are presented, one relies on homogenized specimens while the other utilizes DNA from preservation ethanol, thereby keeping the specimens intact and available for subsequent morphological identification. By parallel processing of samples in 96 well plates, the presented workflow is highly scalable. Additionally, negative and positive controls are introduced to assess reliability of the results. We demonstrate the importance of well-thought out protocols using sequence data from mock communities, and we highlight inherent shortcomings and limitations of metabarcoding. Finally, bioinformatics solutions are briefly presented. This talk will provide you with a good understanding of the basics of metabarcoding. For a more in-depth look, you are invited to participate at the metabarcoding workshop on Sunday!
- Metagenomics
- Invertebrate
- Monitoring
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Presenters/Authors
Vasco Elbrecht
(), Centre for Biodiversity Genomics (University of Guelph), elbrecht@uoguelph.ca;
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Bilgenur Baloglu
(), Centre for Biodiversity Genomics (University of Guelph), bbaloglu@uoguelph.ca;
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Thomas Braukmann
(), Centre for Biodiversity Genomics (University of Guelph), tbraukma@uoguelph.ca;
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Dirk Steinke
(), Centre for Biodiversity Genomics (University of Guelph), dsteinke@uoguelph.ca;
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